Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 23;8:14744. doi: 10.1038/ncomms14744

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a,b) Representative images (single optical planes) of adult (a) and fetal (17 weeks gestation) (b) human skin sections immunolabelled with the indicated antibodies and counterstained with DAPI to reveal nuclei. White dotted lines demarcate dermal-epidermal boundaries. Scale bars, 50 μm. (c) Western blot analysis of NHKs, SCC13 cells and primary cSCC cells (SCC-NR) using antibodies against WBP2. Tubulin was used as loading control. (d,e) Semiquantitative analysis (H-score) of YAP (d) and WBP2 (e) immunostaining intensities (individual data points) in a panel of cSCC- compared with normal skin sections (dotted line). Red lines represent the mean. (f) Western blot analysis of enriched populations of stem- (SC), transit amplifying- (TA), and terminally differentiated (D) cells using antibodies against YAP, WBP2, ITGβ1, and involucrin (IVL). Equal protein loading was confirmed by enhanced tryptophan fluorescence imaging (Bio-Rad) of PVDF membranes (loading control). (g) Representative images (maximum intensity projections) of expanding and mature SC colonies as well as abortive colonies, immunolabelled with indicated antibodies and counterstained for DAPI to reveal nuclei. Scale bars, 100 μm. PVDF, polyvinylidene difluoride.