Table I.

Sequence specific primers

qPCR primers	Sequence
Itch	forward, 5’-TCACTTGGGCATAGGTCTCT-3’
	reverse, 5’-TGTGCCCAGACACTGAGTTA-3’
Wwp1	forward, 5’-AGAATGGAGACCCTGCAACAAG-3’
	reverse, 5’-GCAGGAGTTGGGAACAACAGTA-3’
Smurf1	forward, 5’-AGTTCGTGGCCAAATAGTGG-3’
	reverse, 5’-GTTCCTTCGTTCTCCAGCAG-3’
Smurf2	forward, 5’-TGTAACACAGGCAAATCCCA-3’
	reverse, 5’-CGACGTGGGGACAGATAAAT-3’
RelA	forward, 5’-GCAGTATCCATAGCTTCCAG-3’
	reverse, 5’-GCACTGCATTCAAGTCATAGT-3’
RelB	forward, 5’-TACGACAAGAAGTCCACCA-3’
	reverse, 5’-CACATCAGCTTGAGAGAAGTC-3’
p50	forward, 5’-ACCAAAACAGAGGGGATTT-3’
	reverse, 5’-CCATTTGTGACCAACTGAA-3’
p52	forward, 5’-CTGTCAAGATCTGTAACTATGAGG-3’
	reverse, 5’-ATGTCCTTGGGTCCTACAG-3’
ALP	forward, 5’-CTTGCTGGTGGAAGGAGGCAGG-3’
	reverse, 5’-CACGTCTTCTCCACCGTGGGTC-3’
Runx2	forward, 5’-CAAGAAGGCTCTGGCGTTTA-3’
	reverse, 5’-TGCAGCCTTAAATGACTCGG-3’
RANKL	forward, 5’-CAGAAGGAACTGCAACACAT-3’
	reverse, 5’-CAGAGTGACTTTATGGGAACC-3’
OPG	forward, 5’-AATTGGCTGAGTGTTTTGGT-3’
	reverse, 5’-TGATGTTTCCACAGCTTCAG-3’
GAPDH	forward, 5’-GGTCGGTGTGAACGGATTTG-3’
	reverse, 5’-ATGAGCCCTTCCACAATG-3’