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Primers for genotyping
| Primer type | Sequence |
|---|---|
| Genotyping primers | |
| Itch WT | 5’-ATCGTCTACTCACCCCACATAAGG-3’ |
| Itch KO | 5’-AAGAAGCAGCAGAGACAACGAGTG-3’ |
| Common: | 5’-TCTATGCTCTGTTGTCTCCCATGC-3’ |
| ChIP primers | |
| NF-κB binding site 1 | forward, 5’-GCAGAAATGTCCCAAAGA-3’ |
| reverse, 5’-TGGAAAGCCAGCAAAGC-3’ | |
| NF-κB binding site 2 | forward, 5’-TATGGAGATTATTAGGCTGG TG-3’ |
| reverse, 5’-GAGCGGGGTCTACAAAGT-3’ | |
| Irrelevant site | forward, 5’-GTTCCTACAGTCTAGTTGCATTCA-3’ |
| reverse, 5’-GATGTGGAGGTCAGAGGAC-3’ |
