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. 2017 Mar 24;8:14685. doi: 10.1038/ncomms14685

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Copyright © 2017, The Author(s)

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(a) Schematic illustration of single molecule RNA FISH (smRNA FISH) of Hoxa5 transcripts in vitro and in vivo. (b,c) smRNA FISH of Hoxa5 transcripts on Hb9::GFP ESC-derived motor neurons (b) or on sagittal sections (5–10 μm) of mouse spinal cord of E9.5 Hb9::GFP mouse embryos (c). Scr: scrambled sequence control probe. Scale bar represents 20 μm. (d,e) smRNA FISH signal quantifications show strong cellular variance of Hoxa5 mRNA number in the pMNs (Olig2^on, GFP^off) compared to robust and steady Hoxa5 levels in postmitotic MNs (GFP^on). N-cadherin (NCAD) is used to demarcate cell boundaries and nuclei are counterstained with DAPI. CV: coefficient of variation.