Fig. 3.
Self-organized 3D spheroids upon BMP2 treatment. a 3D VMC-HA hydrogel spheroids after 13-day stimulation with 500 ng/mL of BMP-2, the activator of MGP. b 2.5x magnification of (a). (c) Landscape after removal of the volume above the oblique section plane. d Simulation of a similar 3D morphology of distributed spheroids (D = 0.005, q = 0.003, χ = 0.10, U EX = 8 × 10−6, V EX = 0). Regions of high cell concentration were shown in red. F-actin outlining the cellular skeletons was stained with Alexa Fluor 488 phalloidin and VMC nuclei were stained with DAPI