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. 2017 Apr 3;11:12. doi: 10.1186/s13036-017-0055-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a The mechanical property of the HA hydrogel used in this experiment. b The volume ratio of the multicellular structure to the 3D matrix for the three types of multicellular structures. The cell occupied volume fractions (COVF, mean ± SD) for the three types of multicellular structures had statistically significant difference between each other. ^* P < 0.03 versus the control 3D networks, n = 4; ^# P < 0.001 versus the control 3D networks, n = 4. c Geometric parameter values including the characteristic length of network and the diameter of multicellular spheroid for three experimental groups. The characteristic lengths (mean ± SD) between adjacent network nodes had statistically significant difference in control and 3D thicker network samples. ^** P < 0.05; n =20