Fig. 1.

Example of target area identification and positioning of shadow stick during selection. a Overview of the target area. b–d Target area in close proximity. Red and green indicates K19 and K14 immunofluorescence respectively. The K19⁺/K14⁺ target cells are encircled in (d). e The exact same area on a consecutive neighboring slide. Note that the slide has been inverted in order to position shadow stick above cells. f Positioning of shadow stick during selection