Figure 6.

The 2nd Ana-1 labelled centriole is the PCL. (a) Single 0.3 µm thick confocal sections showing that the proximal Ana1 dot is located inside the nucleus boundary in golfclub/S-shaped spermatid stage (i–iii). Circles mark the location of the centriole and PCL in the inset. Three-dimensional volume model of the PCL in an S-shaped spermatid iii (iv). Scale bars, 1 µm. (b) Serial section TEM analysis showing that only the PCL is found in the cytosolic invagination within the nucleus at the golfclub/S-shape spermatid stage. Scale bars, 200 nm. (c) A schematic diagram showing the location of the PCL in a cytosolic invagination within the nucleus. Abbreviations: Ax, axoneme; C, centriole; N, nucleus; P, PCL.