Fig. 4. Transfection of MCF-7 cells with CRISPR/dCas9, TALEs and ZFs using VP64 effector for the activation of MASPIN. Transfection using polymer 12c resulted in increased MASPIN mRNA (a) across all platforms and increased MASPIN protein (b) levels for CRISPR/dCas9 compared to Lipofectamine 2000 (**p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, ns p > 0.05). Cells were transfected with an empty plasmid as a control.
