Figure EV3. Long‐term Mstn/GDF11 manipulation in vivo .

1. Four‐week‐old C57BL/6 male mice were injected IP with PBS (control; n = 6) or 1 × 10¹² gc of AAV2/8 packaged liver‐specific constructs of full‐length Mstn (AAV8.Mstn; n = 7), Mstn D76A propeptide (AAV8.dnMstn; n = 3), or GDF11 D120A propeptide (AAV8.dnGDF11; n = 4) and euthanized at 16 weeks of age (84 day treatments).
2. Body weights and muscle masses of 12‐week‐old Mstn^WT/WT (n = 6), Mstn^WT/KO (n = 6), and Mstn^KO/KO (n = 4) mice congenic on a C57BL/6 background.

Data Information: Values are presented as mean ± SEM. Statistical analysis performed using one‐way ANOVA analysis with Tukey's HSD post hoc test (non‐connecting letters indicate P < 0.05 between groups) and effect size presented as eta‐squared (η²).