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. 2017 Feb 27;9(4):448–461. doi: 10.15252/emmm.201606932

Figure 2. Gavage of faecal microbiota protects against radiation‐induced death and haematopoietic toxicity.

Figure 2

  • A
    The Shannon diversity index and β‐diversity of intestinal bacteria between male and female mice without irradiation were assessed by 16S high‐throughput sequencing, n = 4. Statistically significant differences are indicated: Student's t‐test. The top and bottom boundaries of each box indicate the 75th and 25th quartile values, respectively, and lines within each box represent the 50th quartile (median) values. Ends of whiskers mark the lowest and highest diversity values in each instance.
  • B
    The relative abundances of the top 10 bacteria at the genus level in male and female mice were assessed using 16S high‐throughput sequencing, n = 4.
  • C
    Scheme for radioprotection after TBI.
  • D
    Male mice received 10 days of oral gavage with saline, male gut microbes, female gut microbes or a male/female gut microbe mixture after 6.5 Gy TBI, Kaplan–Meier survival analysis of the mice was performed. P < 0.005 by log‐rank test between the saline + TBI group and the male + TBI group, P < 0.05 by the log‐rank test between the saline + TBI group and the female + TBI group (or female/male + TBI group), n = 18 per group.
  • E
    Male mice received 10 days of oral gavage with saline, male gut microbes, female gut microbes or a male/female gut microbe mixture after 6.5 Gy TBI, and the body weight was measured. Mean ± SD. Significant differences are shown relative to Saline + TBI group: **P < 0.01, ***P < 0.001; Student's t‐test, n = 18 per group.
  • F, G
    Photographs (F) and volume (G) of dissected spleens from radiation‐exposed male mice, the spleens were obtained at day 15 after 6.5 Gy TBI. Mean ± SD. Significant differences are shown relative to Saline + TBI group: **P < 0.01; Student's t‐test, n = 6 in Saline group, n = 8 in experimental groups.
  • H
    Scheme for faecal microbiota transplantation combined with bone marrow transplant after TBI.
  • I
    Kaplan–Meier survival analysis of the irradiated mice treated with saline, FMT, BMT or FMT combined with BMT was performed. P < 0.005 by log‐rank test between FMT combined with BMT and other three groups, n = 12 per group.