Skip to main content
. 2017 Feb 27;9(4):448–461. doi: 10.15252/emmm.201606932

Figure EV2. Faecal microbiota transplant ameliorates GI tract function and epithelial integrity after irradiation.

Figure EV2

Female mice were separated into two groups after 6.5 Gy gamma ray exposure, one cohort was treated with saline as control, and the other was treated with sex‐matched FMT.
  • A
    The morphology of small intestine in radiation‐induced mice treated with saline or sex‐matched FMT is shown by H&E, AB‐PAS and PAS staining, the small intestine tissues were obtained at day 21 after TBI. The arrows point to the mucus layer or goblet cells.
  • B, C
    Faecal pellet counts removed from cage bedding per 3 day from representative cages. Mean ± SD, n = 6 mice per treatment. ***P < 0.001 by Student's t‐test between Saline + TBI and Female + TBI group.
  • D
    The FITC–dextran in peripheral blood from saline‐treated and sex‐matched FMT mice was assessed at day 21 after irradiation exposure. Mean ± SD. Significant differences are indicated: *P < 0.05, **P < 0.01; Student's t‐test, n = 6 per group.
  • E–I
    The expression levels of Muc2, Glut1, Pgk1, MDR1 and TFF3 were examined in saline‐treated and sex‐matched FMT mice by quantitative PCR, the small intestine tissues were obtained at day 21 after TBI. Mean ± SD. Statistically significant differences are indicated: **P < 0.01; Student's t‐test, n = 12 per group.