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. 2017 Mar 7;36(7):904–918. doi: 10.15252/embj.201693907

Figure 5. PRR proteins physically interact with CO protein in planta .

Figure 5

  • A, B
    Fluorescence resonance energy transfer (FRET) analysis between PRR:CFP and CO:YFP. PRR:CFP and CO:YFP were co‐expressed in A. thaliana protoplasts, and FRET analyses were performed with 20 cells. Statistical significance was calculated using Student's t‐test. *P < 0.01. Error bars indicate standard error within the 20 samples. Scale bars indicate 10 μm.
  • C
    In vivo immunoprecipitation assay with PRR:GFP and CO:HA protein. PRR:GFP and CO:HA were co‐expressed in N. benthamiana, and co‐immunoprecipitation assays performed.
  • D
    The schematic diagram of truncated CO proteins used for co‐IP in (E).
  • E
    TOC1 preferentially binds to the CCT domain in CO. TOC1:HA was co‐infiltrated with YFP fused to truncated versions of CO protein shown in (D) in N. benthamiana, and co‐immunoprecipitation was performed.
Data information: For each experiment, two biological replicates were performed.