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. 2017 Mar 7;36(7):904–918. doi: 10.15252/embj.201693907

Figure EV5. PRRs interact with CO in vivo .

Figure EV5

  • A
    Co‐IP experiment in N. benthamiana, TRB3 protein was used as the negative control in the experiment shown in Fig 5. Agrobacterium carrying either 35S::TOC1:GFP or 35S::TRB3:YFP were co‐infiltrated with that carrying 35S::HA:CO in N. benthamiana leaves. TOC1:GFP and TRB3:YFP proteins were immunoprecipitated with the GFP antibody, and co‐immunoprecipitation of HA:CO protein was checked.
  • B
    FRET between PRR:CFP and CO:YFP in A. thaliana protoplasts. This is a second experiment of the FRET shown in Fig 5A and B. For each construct combination, FRET in 10 protoplasts was analyzed.
  • C, D
    FRET analysis between PRR:CFP and CO:YFP. PRR:CFP and CO:YFP were co‐expressed in N. benthamiana by Agroinfiltration, and FRET analyses were performed. FRET in 30 cells for each construct combination was analyzed for the first experiment. For the second experiment, 15 cells for each combination were analyzed. Scale bars indicate 10 μm.
Data information: Error bars indicate standard error. In (B) and (D), statistical significance was calculated using Student's t‐test; *P < 0.01.