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. 2017 Feb 23;18(4):603–618. doi: 10.15252/embr.201642800

Figure 5. Dgcr8 ablation does not alter Ngn2 levels, but downregulates Ctip2 and Sox5 expression in newborn neurons.

Figure 5

  • A–C
    Immunofluorescence microscopy of coronal cryosections through the dorsal telencephalon of E12.5 WT, Dgcr8 cHET, and Dgcr8 cKO littermate mouse embryos, showing Ngn2 (green) staining.
  • D
    Quantification of the proportion of Ngn2+ cells, expressed as a percentage of the total Hoechst‐stained nuclei (not shown).
  • E–G
    Immunofluorescence microscopy of coronal cryosections through the dorsal telencephalon of E13.5 WT, Dgcr8 cHET, and Dgcr8 cKO littermate mouse embryos, showing Ctip2 (yellow) staining.
  • H
    Quantification of the proportion of Ctip2+ neurons, expressed as a percentage of the total Hoechst‐stained nuclei (not shown).
  • I–K
    Immunofluorescence microscopy of coronal cryosections through the dorsal telencephalon of E13.5 WT, Dgcr8 cHET, and Dgcr8 cKO littermate mouse embryos, showing Sox5 (cyan) staining.
  • L
    Quantification of the proportion of Sox5+ neurons, expressed as a percentage of the total Hoechst‐stained nuclei (not shown).
Data information: Scale bars: 50 μm. Asterisks indicate the ventricular lumen. Solid and dashed lines indicate cortex boundaries. Cortical plate (CP); subventricular zone (SVZ); ventricular zone (VZ). Pyknotic apoptotic nuclei are excluded from all the quantifications. Bars are mean ± SEM of three embryos per condition (18 counted fields per condition). One‐way ANOVA, *P < 0.05; ***P < 0.001; n.s., not significant.