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. 2017 Feb 23;18(4):603–618. doi: 10.15252/embr.201642800

Figure 7. The Microprocessor regulates Tbr1 expression likely by targeting evolutionarily conserved hairpin structures in its transcript.

Figure 7

  • A
    Read counts of experimentally validated miRNAs targeting the Tbr1 transcript in E13.5 Dicer cKO and Dgcr8 cKO cortices.
  • B
    Western blot and quantification of DGCR8, TBR1, and actin expression in N2A cells upon DGCR8 overexpression.
  • C
    Position of the 5 predicted hairpins (HPs) in Tbr1 mRNA coding sequence (ATG‐TAG, black thin segment).
  • C’
    Predicted secondary structures of the 5 HPs (color code as in C).
  • D
    Western blot and quantification of DGCR8, DROSHA, and actin expression in N2A cells upon Dgcr8 or Drosha knockdown.
  • E
    qRT–PCR analysis of endogenous Dgcr8 and Drosha mRNA in N2A cells upon Dgcr8 or Drosha knockdown.
  • F
    N2A cells transfected with Dgcr8, Drosha, or control (NT) siRNAs, respectively, co‐transfected with vectors described in (C) and (C’).
Data information: Mock (Lipo); DGCR8 overexpression (OE); non‐target (NT) siRNA. Bars indicate the variation from the mean (SEM; n = 3 biological replicas), and P‐values are indicated (paired Student's t‐test); *P < 0.05; **P < 0.01; ***P < 0.001.