Figure EV5. A young stroma microenvironment supports the increase in old MPPs in vitro and the decrease in old CMPs in vivo .

• A
  Schematic representation of the experimental setup.
• B
  Concentration of OPN in the co‐culture supernatant of old BM lineage negative onto young, young OPN KO and old endosteal‐enriched stroma population (n = 4).
• C–E
  Number of old LT‐HSCs (C), ST‐HSCs (D) and MPPs (E) Ly5.1⁺ onto young, young OPN KO and old endosteal‐enriched stroma population (n = 4).
• F, G
  Frequency of old LT‐HSCs Ly5.2⁺ Annexin V negative (F) and BrdU⁺ (G) co‐cultured onto young, young OPN KO and old endosteal‐enriched stroma population (n = 4).
• H, I
  Frequency of young (H) and old (I) (Ly5.1⁺) MEPs, CMPs, GMPs and CLPs progenitors in BM cells in young, old and young OPN KO recipients (Ly5.2⁺) mice after 20 weeks upon transplantation. Data are based on six experimental repeats with five recipient mice per group (e.g., n = 25–30 per group).

Data information: Two‐way ANOVA statistic test was used to compare means among the three different groups. Shown are mean values + 1 s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.