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. 2017 Mar 2;36(7):840–853. doi: 10.15252/embj.201694969

Figure EV6. OPN fragments 20–25 kDa re‐polarize old LT‐HSCs.

Figure EV6

  1. OPN fraction D (20–25 kDa) sequence after enzymatic digestion. In blue, the thrombin cleavage site is shown. (ii) Chromatogram of the second digestion of fraction D with 8 M urea.
  2. Western blot analysis of all the fractions obtained from the digestion of fraction D. Antibodies anti‐OPN and anti‐thrombin were used.
  3. All the subfractions were tested on LT‐HSCs. Percentage of LT‐HSCs polarized for AcH4K16 and tubulin are shown for all the experimental groups. n = 3. ˜40 cells scored per sample in each experimental repetition.
  4. Percentage of LT‐HSCs polarized for AcH4K16 and tubulin in the experimental groups listed. n = 3; ˜30 cells scored per sample in each experimental repetition. The percentage of polarized cells is plotted over the total number of cells scored.
Data information: Two‐way ANOVA statistic test was used to compare means among the different groups. Shown are mean values + 1 s.e.m. *P < 0.05, **P < 0.01, **P < 0.001.