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. 2017 Feb 27;18(4):632–644. doi: 10.15252/embr.201642377

Figure 1. Cep78 interacts with Cep76 and CP110, localizes to the distal region of centrioles, and is cell cycle regulated.

Figure 1

  1. Flag or Flag‐Cep78 was expressed in HEK293 cells. Lysates were immunoprecipitated with an anti‐Flag antibody and Western blotted with the indicated antibodies. IN, input. β‐actin was used as a loading control.
  2. RPE‐1 cells expressing Flag‐Cep78 were stained with antibodies against CP110 (red), Flag (green), and C‐Nap1 (blue). Scale bar, 1 μm.
  3. RPE‐1 cells were stained with DAPI (blue) and antibodies against Cep78 (red) and centrin, glutamylated tubulin (GT335), or C‐Nap1 (green). Scale bar, 1 μm.
  4. RPE‐1 cells in different phases of the cell cycle were stained with DAPI (blue) and antibodies against Cep78 (red) and centrin (green). Scale bar, 1 μm.
  5. Cep78 fluorescence intensity at centrosomes was measured and quantitated. At least 75 cells were quantitated in each cell cycle phase and two independent experiments were performed.
  6. RPE‐1 (left) and U2OS (right) lysates from different cell cycle phases were Western blotted with antibodies against Cep78. α‐tubulin was used as a loading control. AS, asynchronous. *denotes full‐length Cep78.

Source data are available online for this figure.