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. 2017 Feb 27;18(4):632–644. doi: 10.15252/embr.201642377

Figure EV1. Characterization of Cep78.

Figure EV1

  1. RPE‐1 cells were stained with DAPI (blue) and antibodies against centrin (green) and Cep78 raised against the C‐terminal region of the protein (IRCM6, red). Scale bar, 1 μm.
  2. RPE‐1 cells untreated or treated with 10 μM nocodazole for 1 h to induce microtubule depolymerization were stained with antibodies against γ‐tubulin (green), α‐tubulin (blue), and Cep78 (red). Scale bar, 1 μm.
  3. RPE‐1 cells transfected with NS siRNA or Cep78 siRNA were stained with DAPI (blue) and antibodies against Cep78 (red) and polyglutamylated tubulin (GT335, green). Scale bar, 1 μm.
  4. RPE‐1 cells were transfected with NS siRNA or Cep78 siRNA (oligo 1, 2 or 6). Lysates were Western blotted with antibody against Cep78. β‐actin was used as a loading control.

Source data are available online for this figure.