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. 2017 Feb 27;18(4):632–644. doi: 10.15252/embr.201642377

Figure 2. Cep78 interacts with EDD‐DYRK2‐DDB1Vpr BP through VprBP .

Figure 2

  1. Flag‐Cep78 protein complexes from HEK293 cells were immunopurified and subjected to mass spectrometric analysis.
  2. Flag or Flag‐Cep78 was expressed in HEK293 cells. Lysates were immunoprecipitated with an anti‐Flag antibody and Western blotted with the indicated antibodies. IN, input.
  3. HEK293 lysates were immunoprecipitated with an irrelevant (control) or anti‐Cep78 antibody and Western blotted with the indicated antibodies. IN, input.
  4. HEK293 cell extracts were chromatographed on a Superpose‐6 column, and the resulting fractions (Fraction‐1 to ‐12) were Western blotted with the indicated antibodies. IN, input.
  5. Purified Cep78 was mixed with purified EDD, DYRK2, DDB1, or VprBP. Proteins were immunoprecipitated with an anti‐Cep78 antibody and Western blotted with the indicated antibodies. IN, input.
  6. Proposed architecture of the Cep78‐bounded EDD‐DYRK2‐DDB1VprBP complex.

Source data are available online for this figure.