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. 2017 Feb 27;18(4):632–644. doi: 10.15252/embr.201642377

Figure EV4. Mapping of interaction domains of Cep78 and EDD‐DYRK2‐DDB1Vpr BP .

Figure EV4

  1. Flag (control), Flag‐Cep78 full‐length (1–722), or Flag‐Cep78 point mutants were expressed in HEK293 cells. Lysates were immunoprecipitated with an anti‐Flag antibody and Western blotted with the indicated antibodies. IN, input.
  2. RPE‐1 cells expressing Flag (control), Flag‐Cep78 full‐length, or point mutants were stained with DAPI (blue) and antibodies against Flag (red) and centrin (green). Scale bar, 1 μm.
  3. Flag (control), Flag‐VprBP full‐length (1–1,507), or Flag‐VprBP truncated mutants were co‐expressed with GFP‐Cep78 in HEK293 cells. Lysates were immunoprecipitated with an anti‐Flag antibody and Western blotted with the indicated antibodies. IN, input.
  4. Myc, Myc‐VprBP full‐length, or Myc‐VprBP truncated mutants were co‐expressed with Flag‐Cep78 in HEK293 cells. Lysates were immunoprecipitated with an anti‐Flag antibody and Western blotted with the indicated antibodies. IN, input. *denotes bands corresponding to the expected proteins.

Source data are available online for this figure.