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. 2017 Feb 27;18(4):632–644. doi: 10.15252/embr.201642377

Figure 6. Cep78 and EDD‐DYRK2‐DDB1Vpr BP control CP110‐dependent centriole elongation in non‐ciliated cells and cilia assembly in ciliated cells.

Figure 6

  • A, B
    HeLa cells transfected with NS siRNA and Cep78 siRNA were stained with DAPI (blue) and antibodies against Cep78 (red) and centrin (green). Scale bar, 1 μm. The percentage of cells with elongated centrioles (centrin filaments) was determined in (B).
  • C, D
    HeLa cells transfected with NS siRNA or Cep97 siRNA and construct expressing an irrelevant Flag‐tagged protein (control), Flag‐Cep78 (1–722), or Flag‐Cep78 (1‐722D290A) were stained with DAPI (blue) and antibodies against Flag (red) and centrin (green). Scale bar, 1 μm. The percentage of cells with elongated centrioles (centrin filaments) was determined in (D).
  • E, F
    HeLa cells transfected with construct expressing an irrelevant Flag‐tagged protein (control), Flag‐EDD, or Flag‐VprBP were stained with DAPI (blue) and antibodies against Flag (red) and centrin (green). Scale bar, 1 μm. The percentage of cells with elongated centrioles (centrin filaments) was determined in (F).
  • G, H
    Quiescent RPE‐1 cells expressing an irrelevant Flag‐tagged protein (control), Flag‐Cep78 (1–722), or Flag‐Cep78 (1‐722D290A) were stained with DAPI (blue) and antibodies against Flag (red) and glutamylated tubulin (GT335, green). Scale bar, 1 μm. The percentage of ciliated cells was determined in (H).
  • I, J
    Proliferating RPE‐1 cells expressing an irrelevant Flag‐tagged protein (control), Flag‐EDD, or Flag‐VprBP were stained with DAPI (blue) and antibodies against Flag (red) and glutamylated tubulin (GT335, green). Scale bar, 1 μm. The percentage of ciliated cells was determined in (J).
Data information: In (B, D, F, H, J) at least 100 cells were scored in each condition and two independent experiments were performed.Source data are available online for this figure.