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. 2017 Feb 20;18(4):586–602. doi: 10.15252/embr.201642140

Figure EV2. NDR1 promotes the IL‐17F‐induced expression of pro‐inflammatory cytokines.

Figure EV2

  • A
    WT and Ndr1‐KO MEFs transfected with a retrovirus encoding mock, Flag‐NDR1, or Flag‐NDR1/K118A were treated with IL‐17 (100 ng/ml) for 0 or 6 h. The mRNA levels of IL‐6, CXCL2, and CCL20 were analyzed by real‐time PCR.
  • B, C
    WT and Ndr1‐KO MEFs (B) or primary astrocytes (C) were treated with IL‐17F (100 ng/ml) for the indicated times, and the induction of IL‐6, CXCL2, CCL20, and CXCL1 mRNA expression was analyzed by real‐time PCR.
  • D
    HeLa cells were transfected with NDR1 siRNA or control siRNA and then were treated with IL‐17F (50 ng/ml) for 0, 1, or 3 h, and the induction of IL‐6, CXCL2, and CCL20 mRNA expression was analyzed by real‐time PCR.
  • E, F
    WT and Ndr1‐KO MEFs were treated with TNF‐α (20 ng/ml) (D) or IL‐1β (10 ng/ml) (E) for the indicated times, and the induction of IL‐6, CXCL2, CCL20, and CXCL1 mRNA expression was analyzed by real‐time PCR.
Data information: *P < 0.05, **P < 0.01 (unpaired, two‐tailed Student's t‐test). Similar results were obtained in at least two independent experiments. Error bars are mean ± SEM values.