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A
HeLa cells were transfected with NDR1 siRNA or control siRNA for 24 h and then were transfected with empty vectors or plasmids encoding Flag‐NDR1 or Flag‐NDR1/K118A for 48 h. The cells were stimulated with 50 ng/ml IL‐17 for 0, 15, 30, or 60 min, and the whole cell lysates were immunoblotted with the indicated antibodies.
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B
HeLa cells were transfected with mock or plasmids encoding Flag‐NDR1 or Flag‐NDR1/K118A and then stimulated with IL‐17 (50 ng/ml) for 0, 15, 30, or 60 min. Whole cell lysates were immunoblotted with the indicated antibodies.
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C
HeLa cells were transfected with NDR1 siRNA or control siRNA and then were treated with IL‐17F (50 ng/ml) for 0, 15, 30, or 60 min. Whole cell lysates were immunoblotted with the indicated antibodies.
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D, E
WT and Ndr1‐KO MEFs (D) or primary astrocytes (E) were treated with IL‐17F (100 ng/ml) for 0, 15, 30, or 60 min. Whole cell lysates were immunoblotted with the indicated antibodies.
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F, G
WT and Ndr1‐KO MEFs were treated with TNF‐α (20 ng/ml) (F) or IL‐1β (10 ng/ml) (G) for 0, 15, 30, or 60 min. Whole cell lysates were immunoblotted with the indicated antibodies.
Data information: Similar results were obtained in three (A, B) or two (C–G) independent experiments.
