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. 2017 Feb 20;18(4):586–602. doi: 10.15252/embr.201642140

Figure 5. NDR1 promotes IL‐17 signaling.

Figure 5

  • A
    HeLa cells were transfected with NDR1 siRNA or control siRNA and then stimulated with 50 ng/ml IL‐17 for 0, 15, 30, or 60 min. Whole cell lysates were immunoblotted with the indicated antibodies.
  • B, C
    WT and Ndr1‐KO MEFs (B) or primary astrocytes (C) were treated with 100 ng/ml IL‐17 for 0, 15, 30, or 60 min. Whole cell lysates were immunoblotted with the indicated antibodies.
  • D
    WT and Ndr1‐KO MEFs transfected with a retrovirus encoding mock, Flag‐NDR1, or Flag‐NDR1/K118A were treated with IL‐17 (100 ng/ml), and the whole cell lysates were immunoblotted with the indicated antibodies.
Data information: Numbers between two blots indicate densitometry of phosphorylated proteins relative to that of total proteins, respectively. Similar results were obtained in at least three (A–C) or two (D) independent experiments.Source data are available online for this figure.