Figure EV5. NDR1 promotes TNF‐α signaling via targeting TRAF3.

1. Wild‐type (Traf3 ^+/+) and TRAF3‐deficient (Traf3 ^−/−) MEFs were transfected with NDR1 siRNA or control siRNA and then stimulated with TNF‐α (20 ng/ml) for the indicated times. Whole lysates were subjected to Western blot.
2. Real‐time PCR analysis of IL‐6, CXCL2, and CCL20 mRNA expression in MEFs treated as described in (A). *P < 0.05 and **P < 0.01 (unpaired, two‐tailed Student's t‐test). Similar results were obtained in two independent experiments. Error bars are mean ± SEM values.

Source data are available online for this figure.