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. 2017 Apr 3;8:462. doi: 10.3389/fpls.2017.00462

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Copyright © Kaur, Pandey, Shivani, Kumar, Pandey, Kesarwani, Mantri, Awasthi and Tiwari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional complementation analysis. (A) Color phenotype of E. coli cultures consisting of pAC-85b (control) and complemented with plasmids containing PSY homologs (NEN-PSY1-3 and RAS-PSY1-3). (B) Concentration of β-carotene in E. coli cells after complementation with plasmids pAC-85b and pTrc containing PSY1-3 of Nendran and Rasthali. Bars denote mean of β-carotene content ± SD. Statistical significance was checked at P ≤ 0.05–0.001 (^** significant; ^*** highly significant) with respect to the Rasthali (PSY1-3).