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. 2015 Jun 1;5:10773. doi: 10.1038/srep10773

Table 1. PmB MIC values of cells expressing ArnT mutant proteins unable to complement the ∆arnT-arnBC + lptG D31H (∆arnT) suppressor mutant.

Residue MIC (μg ml1) Fold difference Presence of l-Ara4N in lipid A
ArnT >1,024 1 Yes
arnT-arnBC+ 0.064 16,000 No
ArnTY43A 0.064 16,000 No
ArnTK69A 0.064 16,000 No
ArnTK69R 0.125 8,192 Yes
ArnTK69E 0.094 10,894 No
ArnTR254A 0.125 8,192 Yes
ArnTR254K 0.064 16,000 Yes
ArnTR254E 0.064 16,000 No
ArnTE493A 0.125 8,192 Yes
ArnTE493D 128 8 Yes
ArnTE493K 0.064 16,000 No

MICs were determined by E-Test. The presence of l-Ara4N in the lipid A was determined by MS-MALDI-TOF using the negative ion mode, as described in Methods.