Table 1. PmB MIC values of cells expressing ArnT mutant proteins unable to complement the ∆arnT-arnBC + lptG D31H (∆arnT) suppressor mutant.
Residue | MIC (μg ml−1) | Fold difference | Presence of l-Ara4N in lipid A |
---|---|---|---|
ArnT | >1,024 | 1 | Yes |
∆arnT-arnBC+ | 0.064 | 16,000 | No |
ArnTY43A | 0.064 | 16,000 | No |
ArnTK69A | 0.064 | 16,000 | No |
ArnTK69R | 0.125 | 8,192 | Yes |
ArnTK69E | 0.094 | 10,894 | No |
ArnTR254A | 0.125 | 8,192 | Yes |
ArnTR254K | 0.064 | 16,000 | Yes |
ArnTR254E | 0.064 | 16,000 | No |
ArnTE493A | 0.125 | 8,192 | Yes |
ArnTE493D | 128 | 8 | Yes |
ArnTE493K | 0.064 | 16,000 | No |
MICs were determined by E-Test. The presence of l-Ara4N in the lipid A was determined by MS-MALDI-TOF using the negative ion mode, as described in Methods.