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. 2015 Jun 1;5:10734. doi: 10.1038/srep10734

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Copyright © 2015, Macmillan Publishers Limited

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(a) The SEC-SAXS/TDA elution profile of Lpn-PlaB. RALS (black), RI (light gray) and the corresponding MW_RALS correlations (gray) across the elution peak. The oligomerization states are indicated and the 3D reconstructions presented. SAXS I(0) values determined from the SEC-SAXS pipeline are shown as open circles. (b+c) Determination of R_g and MW across the SAXS I(0) peak. The MW_RALS (230 ± 15 kDa) corresponds to the MW determined from the zero angle scattering by SAXS (225 ± 15 kDa). Due to weak scattering intensities only the tetrameric peak I(0) was detected automatically by the pipeline. (d) SAXS profiles of Lpn-PlaB collected in batch mode (black) and after component separation (tetramer (gray)) (e) the corresponding 3D reconstructions (inlay, as mesh and bead model, respectively).