Figure 5.

Expression of Nrf2 at the mRNA and protein levels was determined by (A) reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and (B) western blotting, respectively. Isoquercetin increases the phosphorylation of ERK in primary hippocampal neurons. (C) Hippocampal neuron lysates were subjected to western blotting to evaluate Nrf2 levels and the phosphorylation of ERK in the presence and absence of PD98059. (D) The densities of bands representing phosphorylated ERK1/2 and Nrf2 were quantified by densitometric scanning from three independent experiments. GAPDH was used as an internal control. Representative western blots are shown for samples run in duplicate from one of three independent experiments. Graphical data are presented as mean ± standard error of the mean (n=3). *P<0.05 and **P<0.01 vs. the OGD/R group; ^#P<0.05, ^##P<0.01 and ^###P<0. vs. the control group. Nrf2, nuclear factor erythroid 2-related factor 2; ERK, extracellular signal-regulated protein kinase 1 and 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; OGD/R, oxygen-glucose deprivation followed by reoxygenation.