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. 2017 Apr 3;7:45022. doi: 10.1038/srep45022

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Copyright © 2017, The Author(s)

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Drosophila YETI impairs CFPD1 function in HeLa cells (A) DAPI staining of HeLa cells expressing Flag-YETI (left panel) or V5-CFDP1 (right panel); (B) Nuclear localization of V5-CFDP1 (left panel) and Flag-YETI (right panel) proteins. (C) Merges; (D) Western blot analysis of fractionated HeLa cells after V5-Cfdp1/Flag-Yeti transfection. WCE, whole cell extract; S2, cytoplasmic soluble fraction; P3, chromatin-bound fraction. (E) Analysis of mitotic index of HeLa cells. HeLa cells overexpressing V5-CFDP1 fusion protein slightly increases mitotic index, compared to the mock-transfected control. RNAi-mediated depletion of CFDP1 produces about 75% reduction of mitotic index; FLAG-YETI overexpression causes a 60% reduction of mitotic index; (F) For IP assays, protein lysate was prepared from HeLa expressing V5-CFDP1 and FLAG-YETI fusion proteins and immunoprecipitated using a mouse anti-V5 antibody. A FLAG-YETI band is detected in the V5-immunoprecipitate, but not in Ms IgG negative control.