Figure 4.

Myostatin induces tenogenic differentiation of C2C12 cells through the Smad2/3 signaling pathway. Relative tenomodulin and myogenin expression in C2C12 cells treated with myostatin and signaling pathway inhibitors. Cultured C2C12 cells were treated with or without 500 ng·mL⁻¹ of myostatin in the presence or absence of 10 μmol·L⁻¹ of inhibitors of ALK (SB341542), p 38 MAPK (SB203580), or MEK (PD 98059) for 5 days. Values represent the mean relative expression ±SEM. N = 4. *Statistically significant, P < 0.05.