Table 1.
Statistical analysis
| Figure | Normality test | Multiple comparisons test | Combination | Summary | Adjusted P value |
|---|---|---|---|---|---|
| 1D | Shapiro–Wilk normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.7134 |
| Shi/+ vs. MBON‐γ1pedc>Shi | *** | < 0.0001 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | *** | 0.0003 | |||
| 1E | Shapiro–Wilk normality test | Kruskal–Wallis test followed by Dunn's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | > 0.9999 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ns | 0.4292 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | ns | > 0.9999 | |||
| 1F | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.6139 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ** | 0.0018 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | * | 0.0163 | |||
| 1G | Kalmogorov–Smirnov normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.9976 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ** | 0.0015 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | ** | 0.0018 | |||
| 2A | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.7175 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ** | 0.0058 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | * | 0.0398 | |||
| 2B | Kalmogorov–Smirnov normality test | Kruskal–Wallis test followed by Dunn's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.4329 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ns | > 0.9999 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | ns | 0.9912 | |||
| 2C | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.3798 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ns | 0.6398 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | ns | 0.8956 | |||
| 2D | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.9623 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ** | 0.0049 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | ** | 0.008 | |||
| 3B | Shapiro–Wilk normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.6803 |
| Shi/+ vs. MBON‐γ1pedc>Shi | *** | 0.0004 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | ** | 0.0018 | |||
| 3C | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. MBON‐γ1pedc/+ | ns | 0.1224 |
| Shi/+ vs. MBON‐γ1pedc>Shi | ** | 0.0016 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>Shi | *** | < 0.0001 | |||
| 4C | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. R83A12/+ | ns | 0.4911 |
| Shi/+ vs. R83A12 > Shi | * | 0.0317 | |||
| R83A12/+ vs. R83A12 > Shi | ** | 0.0019 | |||
| 4D | Kalmogorov–Smirnov normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. R83A12/+ | ns | 0.9334 |
| Shi/+ vs. R83A12 > Shi | ** | 0.0032 | |||
| R83A12/+ vs. R83A12 > Shi | ** | 0.0066 | |||
| 4E | Shapiro–Wilk normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. R83A12/+ | ns | 0.458 |
| Shi/+ vs. R83A12 > Shi | ns | 0.7599 | |||
| R83A12/+ vs. R83A12 > Shi | ns | 0.1518 | |||
| 4F | Shapiro–Wilk normality test | ANOVA followed by Tukey's multiple comparisons test | Shi/+ vs. R83A12/+ | ns | 0.9007 |
| Shi/+ vs. R83A12 > Shi | * | 0.0284 | |||
| R83A12/+ vs. R83A12 > Shi | ** | 0.0095 | |||
| 5A | D'Agostino & Pearson omnibus normality test | Kruskal–Wallis test followed by Dunn's multiple comparisons test | TH>Shi vs. MBON‐γ1pedc/TH | ns | > 0.9999 |
| TH>Shi vs. MBON‐γ1pedc,TH>Shi | ns | > 0.9999 | |||
| TH>Shi vs. MBON‐γ1pedc>Shi | *** | 0.0003 | |||
| MBON‐γ1pedc/TH vs. MBON‐γ1pedc,TH>Shi | ns | > 0.9999 | |||
| MBON‐γ1pedc/TH vs. MBON‐γ1pedc>Shi | * | 0.0199 | |||
| MBON‐γ1pedc,TH>Shi vs. MBON‐γ1pedc>Shi | ** | 0.0036 | |||
| 5B | D'Agostino & Pearson omnibus normality test | Kruskal–Wallis test followed by Dunn's multiple comparisons test | TH>Shi vs. MBON‐γ1pedc/TH | ns | > 0.9999 |
| TH>Shi vs. MBON‐γ1pedc,TH>Shi | ns | > 0.9999 | |||
| TH>Shi vs. MBON‐γ1pedc>Shi | ** | 0.0014 | |||
| MBON‐γ1pedc/TH vs. MBON‐γ1pedc,TH>Shi | ns | > 0.9999 | |||
| MBON‐γ1pedc/TH vs. MBON‐γ1pedc>Shi | ** | 0.0035 | |||
| MBON‐γ1pedc,TH>Shi vs. MBON‐γ1pedc>Shi | * | 0.0105 | |||
| 5C | D'Agostino & Pearson omnibus normality test | ANOVA followed by Tukey's multiple comparisons test | TH>Shi vs. MBON‐γ1pedc/TH | *** | < 0.0001 |
| TH>Shi vs. MBON‐γ1pedc,TH>Shi | ns | 0.7708 | |||
| TH>Shi vs. MBON‐γ1pedc>Shi | *** | < 0.0001 | |||
| MBON‐γ1pedc/TH vs. MBON‐γ1pedc,TH>Shi | *** | 0.0003 | |||
| MBON‐γ1pedc/TH vs. MBON‐γ1pedc>Shi | ns | 0.9355 | |||
| MBON‐γ1pedc,TH>Shi vs. MBON‐γ1pedc>Shi | *** | 0.0002 | |||
| 5D | Shapiro‐Wilk normality test | ANOVA followed by Tukey's multiple comparisons test | dTrpA1/+ vs. MBON‐γ1pedc/+ | ns | 0.9969 |
| dTrpA1/+ vs. MBON‐γ1pedcC>dTrpA1 | ns | 0.6142 | |||
| MBON‐γ1pedc/+ vs. MBON‐γ1pedc>dTrpA1 | ns | 0.6614 | |||
| 6B | D'Agostino & Pearson omnibus normality test | ANOVA followed by Dunnett's multiple comparisons test | MBON‐γ1pedc>Shi vs. MBON‐γ1pedc,TH>Shi | *** | < 0.0001 |
| MBON‐γ1pedc>Shi vs. MB060B>Shi | *** | 0.0007 | |||
| MBON‐γ1pedc>Shi vs. MBON‐γ1pedc,MB060B>Shi | ns | 0.7626 | |||
| MBON‐γ1pedc>Shi vs. MB504B>Shi | *** | < 0.0001 | |||
| MBON‐γ1pedc>Shi vs. MBON‐γ1pedc,MB504B>Shi | *** | 0.0002 | |||
| 6C | Shapiro–Wilk normality test | ANOVA followed by Dunnett's multiple comparisons test | MBON‐γ1pedc>Shi vs. MB438B>Shi | ** | 0.0033 |
| MBON‐γ1pedc>Shi vs. MB438B/MBON‐γ1pedc | ** | 0.0027 | |||
| MBON‐γ1pedc>Shi vs. MBON‐γ1pedc,MB438B>Shi | ns | 0.3367 |
Statistical analyses of the behavioral experiments are summarized. The figure number (first column), the types of normality test (second column), the types of multiple comparisons test (third column), the data for comparison (fourth column), the significance symbol; *indicates P < 0.05, **indicates P < 0.01, ***indicates P < 0.001 and ns indicates P > 0.05 (fifth column) and the specific P value (sixth column) are indicated. See Materials and methods for details about the type of tests. [Correction added after online publication on 8 March 2017: data for 5D added]. [Corrections added after online publication on 22 March 2017: **** changed to ***].