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. 2017 Apr 3;7:45524. doi: 10.1038/srep45524

Figure 4. Viral transduction does not reduce synaptic counts in the IHC area or the suprathreshold amplitude of ABR wave 1, in either normal or noise-stressed animals.

Figure 4

(A,B) Maximal projections from the 22.6 kHz region immunostained for CtBP2 (red) to reveal the synapses between auditory nerve terminals and IHCs. Scale bar in (B) applies to (A). (C,D) Mean DPOAE and ABR thresholds (±SEMs) for all four experimental groups. (E) Mean ABR wave 1 amplitudes (±SEMs) for suprathreshold levels (60–80 dB SPL). (F,G) Mean counts of OHC and IHC survival for all four experimental groups. (H) Mean IHC synaptic counts (±SEMs) for all four experimental groups. Group sizes were: Normal = 5; AAV-EGFP = 5; Noise only = 5, and Noise + AAV-EGFP, n = 8 in (C–E): Normal = 3; AAV-EGFP = 5; Noise only = 5, and Noise + AAV-EGFP, n = 8 in (F,G): Normal = 3; AAV-EGFP = 5; Noise only = 5, and Noise + AAV-EGFP, n = 7 in (H). Threshold data and hair cells counts for Normal and AAV-EGFP groups are reproduced from Figure 1 for comparison to the noise-stressed groups.