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. 2017 Apr 3;7:45590. doi: 10.1038/srep45590

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Copyright © 2017, The Author(s)

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CWMV infection induced the accumulation of heat shock protein 70s (HSP70s) (A) and inhibition of HSP70s by quercetin treatment reduced the accumulation of CWMV RNAs in plants (B). (A) Western-blotting analysis of HSP70 in CWMV-infected Nicotiana benthamiana (top line) and Triticum aestivum (bottom line) using an anti-HSP70 antibody. Rubisco stained with Coomassie brilliant blue (CBB) was used for the loading controls. (B) Northern blotting assay of CWMV RNA accumulation in N. benthamiana plants treated with various concentrations of quercetin. Ethidium bromide (EtBr)-stained rRNAs are shown as the loading controls. Treatment with DMSO was chosen as the control. sgRNA: subgenomic RNA. (C) Symptoms developed at 4 dpi on quercetin- and DMSO-treated N. benthamiana plants.