Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 3;7:45590. doi: 10.1038/srep45590

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Symptoms in N. benthamiana plants infiltrated with the tobacco rattle virus (TRV) vector harboring a partial fragment of N. benthamiana HSP70 (TRV:NbHSP70). The empty vector (TRV:00) was infiltrated and used as a control. Pictures were taken at 7 dpi. (B) qPCR analysis showing the expression levels of NbHSP70 in the N. benthamiana infiltrated with TRV:NbHSP70 compared with the control (TRV:00). Experiments were repeated three times. Bars represent the standard errors of the means. (C) Accumulation of CWMV RNAs analyzed by Northern blotting. Total RNAs were extracted from the inoculated leaves at 4 dpi. Ethidium bromide (EtBr)-stained rRNAs are shown as loading controls. (D) Accumulation of CWMV RNA1 analyzed by Northern blotting using an RNA1-specific probe. Lane 1, the total RNA extracted from a plant inoculated with CWMV RNA 1 and RNA 2. Lane 2, the total RNA extracted from the mock inoculated N. benthamiana. Lane 3, the total RNA extracted from the silencing plants co-inoculated with CWMV RNA1 and 35S:NbHSP70. Lane 4, the total RNA extracted from the control plants inoculated with TRV:00 and CWMV RNA1. Lane 5, the total RNA extracted from the NbHSP70 silenced plants inoculated with CWMV RNA1. Lane 6, the total RNA extracted from the plants inoculated with CWMV RNA1 only. Total RNAs were extracted from the inoculated leaves 4 dpi after inoculation. Ethidium bromide (EtBr)-stained rRNAs are shown as loading controls.