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. 2017 Apr 3;7:45590. doi: 10.1038/srep45590

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Copyright © 2017, The Author(s)

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(A) Seven truncated mutants, covering the three conserved domains (Met, Hel and RdRp) of the CWMV replicase, were designed for assays. The numbers denote CWMV replicase amino acid positions. The ability of CWMV replicase fragments to interact with TaHSP70 or NbHSP70 in YTH assays is shown on the right (+, positive; −, negative). (B) Yeast colonies expressing BD-TaHSP70 with AD-Rep^1–670 and AD-Rep^167–333 grew well on the selective medium, but those expressing AD-Rep^670–1430, AD-Rep^1430–1840, AD-Rep^1–167, AD-Rep^333–670 or AD-MP with BD-TaHSP70 did not. (C) Yeast colonies expressing BD-NbHSP70 with AD-Rep^1–670 and AD-Rep^167–333 grew well on the selective medium, but those expressing AD-Rep^670–1430, AD-Rep^1430–1840, AD-Rep^1–167, AD-Rep^333–670 or AD-MP with BD-NbHSP70d did not. Yeast co-transformed with BD-53 and AD-T, and BD-Lam and AD-T were used as the positive and negative controls, respectively.