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. 2014 Oct 9;4:6563. doi: 10.1038/srep06563

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(a) Representative immunohistochemistry of kidney sections for collagen I (a–d) and αSMA (e–h) (b, c) Comparison by quantifying the positive staining area for collagen I (b) and αSMA (c). (d, e) Real-time PCR for Col1a1 (d) and αSMA (e). A vitamin D3 free diet significantly enhances UUO-induced protein and mRNA expression of collagen I and αSMA in kl/kl mice. Data are presented as the means ± SD. *P < 0.05, #p < 0.05 vs. sham-treated kidneys, &p < 0.05 vs. D-diet kl/kl sham-treated kidneys (n = 5).