Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 9;4:6563. doi: 10.1038/srep06563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014, Macmillan Publishers Limited. All rights reserved

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Representative immunofluorescence for phosphorylated (p)-Smad3. (b) Western blot for pSmad3 in UUO- or sham-treated kidneys. Full-length blots are included in the supplementary information. (c) Comparison of the number of pSmad3-positive nuclei. (d) Comparison of pSmad3 to total Smad3 ratio in western blots. (e) TGF-β1 protein expression in the kidneys as measured by ELISA. (f) TGF-β1 mRNA expression as measured by real-time PCR. Amelioration of UUO-induced activation of TGF-β1 signaling in kl/kl mice was abolished by a vitamin D3 free diet. pSmad3 positive nuclei and TGF-β1 expression were increased at the protein and mRNA levels. Data are presented as the means ± SD. *P < 0.05 (n = 5), #p < 0.05 vs. sham treated kidneys. &P < 0.05 vs. D-diet kl/kl mice.