Table 1. Differentially expressed cytoskeleton, actin cytoskeleton, muscle fibre development, collagen, and calcium-ion-binding proteins.
| Protein description | Gene symbol | Accession | Ratio | p value |
|---|---|---|---|---|
| Actin cytoskeleton | ||||
| PREDICTED: catenin alpha-1-like | ctnna1 | gi|410914176 | 3.499 | 0.036 |
| Glycogen synthase 2 | gys2 | gi|66392235 | 0.064 | 0.049 |
| PREDICTED: myosin heavy chain, fast skeletal muscle, partial | mhc | gi|410932313 | 0.370 | 0.027 |
| Slow myosin heavy chain 1 | smyhc1 | gi|87116414 | 0.236 | 0.025 |
| PREDICTED: talin-2-like | tln2 | gi|410912160 | 4.742 | 0.004 |
| Novel slow skeletal troponin T family protein | tnnt | gi|55962571 | 0.163 | 0.022 |
| Tropomyosin beta chain | tpm2 | gi|50344894 | 0.194 | 0.014 |
| Cytoskeleton | ||||
| PREDICTED: catenin alpha-1-like | ctnna1 | gi|410914176 | 3.499 | 0.036 |
| Glycogen synthase 2 | gys2 | gi|66392235 | 0.064 | 0.049 |
| Keratin 18 | krt18 | gi|380449877 | 0.236 | 0.004 |
| PREDICTED: myosin heavy chain, fast skeletal muscle, partial | mhc | gi|410932313 | 0.370 | 0.027 |
| Myosin-binding protein C, slow-type | mybpc1 | gi|55925504 | 0.417 | 0.000 |
| Slow myosin heavy chain 1 | smyhc1 | gi|87116414 | 0.236 | 0.025 |
| PREDICTED: talin-2-like | tln2 | gi|410912160 | 4.742 | 0.004 |
| Novel slow skeletal troponin T family protein | tnnt | gi|55962571 | 0.163 | 0.022 |
| Tropomyosin beta chain | tpm2 | gi|50344894 | 0.194 | 0.014 |
| Tubulin, alpha 8 like | tuba8l | gi|37362304 | 2.399 | 0.033 |
| Novel protein similar to SPEG complex locus (speg) | ENSDARG00000009567 | gi|169154737 | 0.488 | 0.017 |
| Muscle actin type 1 | zgc:86725 | gi|291167458 | 0.215 | 0.038 |
| Myosin-binding protein C, fast type a | zgc:136545 | gi|113951767 | 2.014 | 0.015 |
| Muscle fibre development | ||||
| Novel protein similar to vertebrate nebulin | neb | gi|94732977 | 0.353 | 0.000 |
| Collagen | ||||
| Collagen, type I, alpha 1b precursor | col1a1p | gi|41393113 | 0.457 | 0.002 |
| Calcium ion binding | ||||
| Annexin 11a isoform 2 | anxa11 | gi|34536830 | 2.312 | 0.025 |
| Calreticulin | crt | gi|312603155 | 0.180 | 0.013 |
| Myosin, light chain 4 | MYL4 | gi|41055823 | 0.217 | 0.002 |
| Myosin, light chain 10, regulatory | myl10 | gi|62955715 | 0.167 | 0.005 |
| Phospholipase C-beta 1 | plcb1 | gi|208342308 | 2.443 | 0.038 |
| Troponin C type 1b (slow) | tnnc1b | gi|50344824 | 0.172 | 0.001 |
The proteins sharing similar biological functions were clustered based on similar trends of differential expression. The table lists the protein description, gene symbol, accession number, ratios and p values of protein expression. Ratio = (protein reporter ion signal intensity of a protein in the muscle of crisp grass carp)/(protein reporter ion signal intensity of the protein in the muscle of grass carp). The p value, representing the probability that the observed ratio is different from 1 by chance, was calculated according to the intensity values of effective peptides in each protein, and using the Student t-test method, where n represents the number of effective peptides per protein. Ratios were deemed to signify differential expression (p < 0.05). These proteins were considered to show a significant upward or downward trend when their expression ratios were <0.5 or >2, respectively.