Table 2. Differentially expressed oxidoreductase activity, biosynthetic process, catalytic activity, transferase activity, and ATP-binding proteins.
| Protein description | Gene symbol | Accession | Ratio | p value |
|---|---|---|---|---|
| Oxidoreductase activity | ||||
| Aldo-ketoreductase family 1, member B1 | akr1b1 | gi|50344750 | 0.474 | 0.004 |
| Aldehyde dehydrogenase 9 family, member A1b | aldh9a1b | gi|161784297 | 87.902 | 0.019 |
| Aldehyde dehydrogenase 1 family, member L1 | aldh1l1 | gi|311771613 | 0.179 | 0.000 |
| 3-Hydroxybutyrate dehydrogenase, type 1 | bdh1 | gi|147899736 | 0.050 | 0.049 |
| RecName: Full = 3-hydroxybutyrate dehydrogenase type 2 | bdh2 | gi|82193321 | 0.084 | 0.029 |
| NADH-cytochrome b5 reductase 2 | cyb5r2 | gi|113679449 | 0.391 | 0.030 |
| Cytochrome P450, family 20, subfamily A, polypeptide 1 | cyp20a1 | gi|47086981 | 0.043 | 0.040 |
| Cytochrome P450 2AD3 | cyp2ad3 | gi|46243655 | 0.461 | 0.043 |
| Uncharacterised protein LOC564675 | ENSDARG00000043403 | gi|165972501 | 0.061 | 0.012 |
| PREDICTED: fatty aldehyde dehydrogenase-like | faldh | gi|410915226 | 0.316 | 0.003 |
| PREDICTED: fatty acid synthase-like | fas | gi|410930382 | 2.228 | 0.031 |
| Glucose-6-phosphate 1-dehydrogenase | g6pd | gi|213512060 | 0.027 | 0.048 |
| Glycerol-3-phosphate dehydrogenase 1b | gpd1b | gi|229366800 | 9.638 | 0.042 |
| PREDICTED: glutathione reductase, mitochondrial-like | gsrm | gi|410914397 | 0.137 | 0.042 |
| Hydroxyacid oxidase 2 | hao2 | gi|41053573 | 0.233 | 0.049 |
| 4-Hydroxyphenylpyruvate dioxygenase a; Key enzyme in the degradation of tyrosine | hpda | gi|82187435 | 0.205 | 0.011 |
| Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase | hsd3b7 | gi|44890388 | 12.589 | 0.028 |
| NAD(P)-dependent steroid dehydrogenase-like | nsdhl | gi|62955325 | 0.156 | 0.014 |
| Phenylalanine hydroxylase | pah | gi|41054599 | 0.083 | 0.044 |
| Pyruvate dehydrogenase (lipoamide) alpha 1a | pdha1a | gi|53749653 | 0.182 | 0.045 |
| Protein disulfideisomerase associated 4 | pdia4 | gi|46361720 | 0.215 | 0.022 |
| Phytanoyl-CoA dioxygenase domain-containing protein 1 | phyhd1 | gi|226358555 | 0.143 | 0.035 |
| Peroxiredoxin 6 | prdx6 | gi|55251344 | 0.112 | 0.005 |
| PREDICTED: xanthine dehydrogenase/oxidase | xdh | gi|189530915 | 0.011 | 0.048 |
| Uncharacterised protein LOC393297 | zgc:56585 | gi|41056185 | 0.138 | 0.031 |
| Oxidoreductase activity, acting on the aldehyde or oxo group of donors | ||||
| Aldehyde dehydrogenase 9 family, member A1b | aldh9a1b | gi|161784297 | 87.902 | 0.019 |
| Aldehyde dehydrogenase 1 family, member L1 | aldh1l1 | gi|311771613 | 0.179 | 0.000 |
| PREDICTED: fatty aldehyde dehydrogenase-like | faldh | gi|410915226 | 0.316 | 0.003 |
| Pyruvate dehydrogenase alpha 1a | pdha1a | gi|53749653 | 0.182 | 0.045 |
| Oxidoreductase activity, acting on the CH–OH group of donors, NAD or NADP as acceptor | ||||
| RecName: Full=3-hydroxybutyrate dehydrogenase type 2 | bdh2 | gi|82193321 | 0.084 | 0.029 |
| PREDICTED: fatty acid synthase-like | fas | gi|410930382 | 2.228 | 0.031 |
| Glucose-6-phosphate 1-dehydrogenase | g6pd | gi|213512060 | 0.027 | 0.048 |
| Glycerol-3-phosphate dehydrogenase 1b | gpd1b | gi|229366800 | 9.638 | 0.042 |
| Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase | hsd3b7 | gi|44890388 | 12.589 | 0.028 |
| NAD(P) dependent steroid dehydrogenase-like | nsdhl | gi|62955325 | 0.156 | 0.014 |
| PREDICTED: xanthine dehydrogenase/oxidase | xdh | gi|189530915 | 0.011 | 0.048 |
| Single-organism biosynthetic process | ||||
| RecName: Full=3-hydroxybutyrate dehydrogenase type 2 | bdh2 | gi|82193321 | 0.084 | 0.029 |
| NADH-cytochrome b5 reductase 2 | cyb5r2 | gi|113679449 | 0.391 | 0.030 |
| RecName: Full=glycine amidinotransferase, mitochondrial | gatm | gi|82187306 | 0.177 | 0.012 |
| Glycogen synthase 2 | gys2 | gi|66392235 | 0.064 | 0.049 |
| Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase | hsd3b7 | gi|44890388 | 12.589 | 0.028 |
| Isopentenyl-diphosphate delta-isomerase 1 | idi1 | gi|70887565 | 0.215 | 0.038 |
| NAD(P)-dependent steroid dehydrogenase-like | nsdhl | gi|62955325 | 0.156 | 0.014 |
| Pyruvate carboxylase | pc | gi|300641377 | 0.053 | 0.009 |
| Pyruvate carboxylase, like | pcl | gi|83415094 | 0.023 | 0.033 |
| Sigma non-opioid intracellular receptor 1 | sigmar1 | gi|82209697 | 0.316 | 0.022 |
| Lipid biosynthetic process | ||||
| NADH-cytochrome b5 reductase 2 | cyb5r2 | gi|113679449 | 0.391 | 0.030 |
| Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase | hsd3b7 | gi|44890388 | 12.589 | 0.028 |
| Isopentenyl-diphosphate delta-isomerase 1 | idi1 | gi|70887565 | 0.215 | 0.038 |
| NAD(P)-dependent steroid dehydrogenase-like | nsdhl | gi|62955325 | 0.156 | 0.014 |
| Sigma non-opioid intracellular receptor 1 | sigmar1 | gi|82209697 | 0.316 | 0.022 |
| Catalytic activity | ||||
| DIP2 disco-interacting protein 2 homolog B | dip2ba | gi|82185659 | 0.156 | 0.041 |
| 26 S proteasome non-ATPase regulatory subunit 2 | psmd2 | gi|41054527 | 3.767 | 0.001 |
| Acyl-CoA synthetase long-chain family member 1 | zgc:101071 | gi|57525836 | 0.242 | 0.023 |
| Transferase activity | ||||
| Acetyl-coenzyme A acyltransferase 1 | acaa1 | gi|50345072 | 0.146 | 0.045 |
| Glutathione S-transferase M | gstm | gi|218455223 | 2.312 | 0.008 |
| Glutathione S-transferase pi 1 | gstp1 | gi|218455225 | 3.733 | 0.023 |
| N-Myristoyltransferase 1a | nmt1a | gi|47217841 | 0.071 | 0.006 |
| Rho-class glutathione-S-transferase | rgst | gi|190410767 | 0.018 | 0.025 |
| Cytosolic sulfotransferase 2 | sult2st2 | gi|148745693 | 0.108 | 0.042 |
| ATP binding | ||||
| Aldo-ketoreductase family 1, member B1 | akr1b1 | gi|50344750 | 0.474 | 0.004 |
| Chaperonin containing TCP1, subunit 5 | cct5 | gi|49619081 | 2.421 | 0.018 |
| Novel protein similar to SPEG complex locus (speg) | ENSDARG00000009567 | gi|169154737 | 0.488 | 0.017 |
| 60 kDa heat shock protein 1 | hspd1 | gi|315585122 | 0.126 | 0.008 |
| PREDICTED: isoleucine–tRNA ligase, mitochondrial-like | ltlm | gi|410925971 | 4.875 | 0.031 |
| PREDICTED: myosin heavy chain, fast skeletal muscle, partial | mhc | gi|410932313 | 0.370 | 0.027 |
| Pyruvate carboxylase | pc | gi|300641377 | 0.053 | 0.009 |
| Pyruvate carboxylase-like | pcl | gi|83415094 | 0.023 | 0.033 |
| Proteasome (prosome, macropain) 26 S subunit, ATPase, 3 | psmc3 | gi|50344782 | 2.148 | 0.049 |
| Slow myosin heavy chain 1 | smyhc1 | gi|87116414 | 0.236 | 0.025 |
| Muscle actin type 1 | zgc:86725 | gi|291167458 | 0.215 | 0.038 |
The proteins sharing similar biological functions were clustered based on similar trends of differential expression. The table lists the protein description, gene symbol, accession number, ratios and p values of protein expression. Ratio = (protein reporter ion signal intensity of a protein in the muscle of crisp grass carp)/(protein reporter ion signal intensity of the protein in the muscle of grass carp). The p value, representing the probability that the observed ratio is different from1 by chance, was calculated according to the intensity values of effective peptides in each protein, and using the Student t-test method, where n represents the number of effective peptides per protein. Ratios were deemed to signify differential expression (p < 0.05). These proteins were considered to show a significant upward or downward trend when their expression ratios were <0.5 or >2, respectively.