Table 3. Differentially expressed membrane, nucleus, ribosome, transporter activity and other GO proteins.
| Protein description | Gene symbol | Accession | Ratio | p value |
|---|---|---|---|---|
| Membrane | ||||
| Complement component C9 | ccc9 | gi|126045475 | 2.831 | 0.038 |
| GTP cyclohydrolase I feedback regulator | gchfr | gi|62286827 | 8.241 | 0.042 |
| Stomatin-like protein 2 | stom2 | gi|223648686 | 0.104 | 0.021 |
| Troponin C type 1b (slow) | tnnc1b | gi|50344824 | 0.172 | 0.001 |
| Nucleus | ||||
| DIP2 disco-interacting protein 2 homolog Ba | dip2ba | gi|82185659 | 0.156 | 0.041 |
| Unnamed protein product | no | gi|311812832 | 0.134 | 0.030 |
| Chaperone protein promoting assembly of the 20 S proteasome as part of a heterodimer with psmg1 | psmg2 | gi|94732190 | 0.018 | 0.047 |
| Ribosome | ||||
| Ribosomal protein L13 | rpl13 | gi|44966222 | 0.081 | 0.021 |
| 40 S ribosomal protein S13 | rps13 | gi|50344812 | 0.156 | 0.010 |
| Transporter activity | ||||
| Archain 1b | arcn1b | gi|49902633 | 3.565 | 0.044 |
| Brain-type fatty-acid-binding protein 7a | fabp7a | gi|8809798 | 0.325 | 0.040 |
| PREDICTED: importin-4 | ipo4 | gi|326679575 | 0.180 | 0.038 |
| N-Ethylmaleimide-sensitive fusion protein attachment protein alpha | napa | gi|41054285 | 5.297 | 0.029 |
| Unnamed protein product | no | gi|47224597 | 7.178 | 0.039 |
| Solute carrier family 25 alpha, member 5 | slc25a5 | gi|41107664 | 0.081 | 0.039 |
| Other GOs | ||||
| Adenosylhomocysteinase | ahcy | gi|40363541 | 0.093 | 0.008 |
| Beta-2-microglobulin | b2m | gi|57281691 | 0.084 | 0.046 |
| PREDICTED: coiled-coil domain-containing protein 47-like | ccdc | gi|410902989 | 0.340 | 0.046 |
| Chymotrypsin B1 precursor | ctrb1 | gi|47086795 | 0.102 | 0.036 |
| D-Dopachrometautomerase | ddt | gi|50344950 | 0.077 | 0.003 |
| REDICTED: elongator complex protein 1 | elp5 | gi|348511894 | 6.486 | 0.034 |
| Family with sequence similarity 82, member B | FAM82B | gi|157311701 | 0.012 | 0.038 |
| Fumarylacetoacetase | fase | gi|41054569 | 0.223 | 0.042 |
| Fibrinogen gamma polypeptide | fgg | gi|295314912 | 0.273 | 0.003 |
| Haemoglobin alpha adult-1 | hbaa1 | gi|22135554 | 0.064 | 0.009 |
| PREDICTED: low-quality protein: heat repeat-containing protein 3-like | hrcp3 | gi|410912234 | 2.168 | 0.037 |
| Interferon-inducible protein Gig1 | ifnip | gi|31580624 | 12.706 | 0.036 |
| Karyopherin (importin) beta 3 | kpnb3 | gi|348536891 | 2.128 | 0.039 |
| PREDICTED: hypothetical protein LOC100691952 | loc100691952 | gi|348539826 | 0.163 | 0.047 |
| PREDICTED: uncharacterized protein LOC101068541 | loc101068541 | gi|410918305 | 0.219 | 0.044 |
| PREDICTED: bifunctional protein NCOAT-like | LOC571547 | gi|326672078 | 0.053 | 0.010 |
| PREDICTED: hypothetical protein LOC794259 | loc794259 | gi|292619409 | 6.427 | 0.034 |
| Metallothionein II | mt2 | gi|7579035 | 0.043 | 0.039 |
| PREDICTED: pleckstrin homology domain-containing family A member 6-like | PLEKHA6 | gi|292618909 | 0.229 | 0.040 |
| Peptidyl-prolyl cis-trans isomerase H | ppih | gi|225707560 | 2.089 | 0.024 |
| Protein TFG | tfg | gi|41053365 | 3.837 | 0.041 |
| Tetratricopeptide repeat domain 36 | ttc36 | gi|187471140 | 0.082 | 0.008 |
The proteins sharing similar biological functions were clustered based on similar trends of differential expression. The table lists the protein description, gene symbol, accession number, ratios and p values of protein expression. Ratio = (protein reporter ion signal intensity of a protein in the muscle of crisp grass carp)/(protein reporter ion signal intensity of the protein in the muscle of grass carp). The p value, representing the probability that the observed ratio is different from 1 by chance, was calculated according to the intensity values of effective peptides in each protein, and using the Student t-test method, where n represents the number of effective peptides per protein. Ratios were deemed to signify differential expression (p < 0.05). These proteins were considered to show a significant upward or downward trend when their expression ratios were <0.5 or >2, respectively.