Figure 6. Butyrate treatment increased the sensitivity of Caco-2 cells to Stx1.

Caco-2 cells were cultured with 2 mM sodium butyrate-containing medium or normal medium for 4 days. The cells were then exposed to various concentrations of Stx1 (0, 10, 30, 100 or 300 pg/mL) for 48 h. The viable cells were quantitated by means of WST-8 cell viability assaying. Data for butyrate-treated cells (open circles) and untreated cells (open triangles) are shown. Viability was calculated as the relative value to that without toxin exposure. Data are expressed as means ± SD of triplicate determinations. Error bars underneath the symbols are not visible.