FIG 3.
Toxicity of the ΦM1 escape locus products. (A) The escape locus of ΦM1 as described in the legend to Fig. 1B. The positions of the ΦM1 escape phage mutations are shown by red vertical lines, labeled with the parent phage. The scale is in base pairs. (B) Specific regions of the phage genomes, designated by the length of the line that corresponds to the genomic locus shown in panel A, were cloned into pBAD30 to make nine different constructs. Blue dashed lines in panel A reflect the construct boundaries in panel B. The figure is drawn to scale. (C) Expression of ΦM1 wt and ΦM1-B escape loci in P. atrosepticum. Strains of P. atrosepticum containing either a ToxINPa or ToxINPa-FS plasmid (pTRB125 or pTRB126), together with a phage construct (or pBAD30 vector control), were tested for toxicity. (D) A range of construct 7 plasmids was tested for toxicity in P. atrosepticum. The escape phage constructs were all reduced for toxicity. Error bars show the standard deviations for triplicate data.