TABLE 3.
Bacterial strains and bacteriophages used in this study
| Bacterium or phage | Genotype or characteristics | Reference or source |
|---|---|---|
| Bacteria | ||
| Escherichia coli β2163 | F− RP4-2-Tc::Mu dapA::(erm-pir) Kmr | 57 |
| E. coli DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK+) λ− | Gibco/BRL |
| E. coli ER2566 | F− λ− fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10) (TetS) endA1 [dcm] | NEB |
| E. coli W3100 | F− λ− rph-1 INV(rrhD, rrhE) | 58 |
| Pectobacterium atrosepticum SCRI1043 | Wild-type strain | 35 |
| Phages | ||
| ΦM1 | Podoviridae, propagated on wt SCRI1043 | 30 |
| ΦM1-A | ToxINPa escape mutant of ΦM1 | 29 |
| ΦM1-B | ToxINPa escape mutant of ΦM1 | 29 |
| ΦM1-C | ToxINPa escape mutant of ΦM1 | 29 |
| ΦM1-D | ToxINPa escape mutant of ΦM1 | 29 |
| ΦM1-O | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-V | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-W | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-X | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-Y | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-Z | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-Q | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-E1 to -E49 | ToxINPa escape mutant of ΦM1 | This study |
| ΦM1-U1 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U2 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U4 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U5 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U6 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U7 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U8 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U9 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-U10 | ToxINPa escape mutant of ΦM1 on UvrA mutant | This study |
| ΦM1-PL2 | TenpINPl escape mutant of ΦM1 | This study |