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. 2017 Mar 31;83(8):e00028-17. doi: 10.1128/AEM.00028-17

TABLE 1.

Concentrations of fecal indicator bacteria, bacterial and viral microbial source tracking markers, and enteric viruses per 3 μl of DNA/RNA extracted from beach water samples seeded with raw wastewater

Dilution and corresponding amt of raw wastewater into beach water samples Concn (mean ± SD) (per 3 μl of DNA/RNA) ina:
ENT 23S (FIB) Bacterial or viral MST marker
Enteric virus
EC H8 HF183 nifH HAdV HPyV PMMoV HAdV 40/41 HNoV EV
3 ml 3.3 × 105 ± 1.3 × 105 4.2 × 105 ± 5.2 × 104 1.0 × 106 ±1.1 × 105 2.7 × 103 ± 8.3 × 102 2.0 × 103 ± 3.7 × 102 4.4 × 104 ± 5.8 × 103 2.6 × 103 ± 2.8 × 102 3.1 × 102 ± 1.7 × 102 <PLOQ 5.7 ×102 ± 2.6 ×102
10−1 (300 μl) 6.1 × 104 ± 1.3 × 104 4.3 × 104 ± 9.8 × 103 2.2 × 105 ± 6.0 × 104 3.5 × 102 ± 6.0 × 101 2.3 × 102 ± 1.5 × 102 7.5 × 103 ± 6.8 × 103 6.0 × 102 ± 1.6 × 102 9.4 × 101 ± 4.1 × 101 <PLOD 7.0 ×101 ± 3.1 ×101
10−2 (30 μl) 2.8 × 103 ± 2.3 × 103 1.6 × 103 ± 1.1 × 103 1.1 × 104 ± 7.4 × 103 1.3 × 101 ± 1.2 × 101 1.1 × 101 ± 9.9 × 100 8.0 × 102 ± 3.2 × 101 1.4 × 102 ± 5.2 × 101 <PLOD <PLOD <PLOD
10−3 (3 μl) 5.7 × 102 ± 3.7 × 102 3.8 × 102 ± 9.3 × 101 2.9 × 103 ± 8.1 × 102 <PLOD <PLOD 9.1 × 101 ± 1.4 × 101 4.9 × 101 ± 2.4 × 101 <PLOD <PLOD <PLOD
10−4 (300 nl) 1.3 × 102 ± 4.4 × 101 1.0 × 102 ± 4.3 × 101 6.7 × 102 ± 3.3 × 102 <PLOD <PLOD <PLOD <PLOD <PLOD <PLOD <PLOD
10−5 (30 nl) 3.2 × 101 ± 1.5 × 101 <PLOD 2.9 × 102 ± 2.1 × 102 <PLOD <PLOD <PLOD <PLOD <PLOD <PLOD <PLOD
10−6 (3 nl) <PLOD <PLOD 5.2 × 101 ± 7.9 × 101 <PLOD <PLOD <PLOD <PLOD <PLOD <PLOD <PLOD
a

FIB, fecal indicator bacteria; MST, microbial source tracking; PLOQ, process limit of quantification; PLOD, process limit of detection.

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