TABLE 1.

Concentrations of fecal indicator bacteria, bacterial and viral microbial source tracking markers, and enteric viruses per 3 μl of DNA/RNA extracted from beach water samples seeded with raw wastewater

Dilution and corresponding amt of raw wastewater into beach water samples	Concn (mean ± SD) (per 3 μl of DNA/RNA) ina:
	ENT 23S (FIB)	Bacterial or viral MST marker						Enteric virus
		EC H8	HF183	nifH	HAdV	HPyV	PMMoV	HAdV 40/41	HNoV	EV
3 ml	3.3 × 105 ± 1.3 × 105	4.2 × 105 ± 5.2 × 104	1.0 × 106 ±1.1 × 105	2.7 × 103 ± 8.3 × 102	2.0 × 103 ± 3.7 × 102	4.4 × 104 ± 5.8 × 103	2.6 × 103 ± 2.8 × 102	3.1 × 102 ± 1.7 × 102	<PLOQ	5.7 ×102 ± 2.6 ×102
10−1 (300 μl)	6.1 × 104 ± 1.3 × 104	4.3 × 104 ± 9.8 × 103	2.2 × 105 ± 6.0 × 104	3.5 × 102 ± 6.0 × 101	2.3 × 102 ± 1.5 × 102	7.5 × 103 ± 6.8 × 103	6.0 × 102 ± 1.6 × 102	9.4 × 101 ± 4.1 × 101	<PLOD	7.0 ×101 ± 3.1 ×101
10−2 (30 μl)	2.8 × 103 ± 2.3 × 103	1.6 × 103 ± 1.1 × 103	1.1 × 104 ± 7.4 × 103	1.3 × 101 ± 1.2 × 101	1.1 × 101 ± 9.9 × 100	8.0 × 102 ± 3.2 × 101	1.4 × 102 ± 5.2 × 101	<PLOD	<PLOD	<PLOD
10−3 (3 μl)	5.7 × 102 ± 3.7 × 102	3.8 × 102 ± 9.3 × 101	2.9 × 103 ± 8.1 × 102	<PLOD	<PLOD	9.1 × 101 ± 1.4 × 101	4.9 × 101 ± 2.4 × 101	<PLOD	<PLOD	<PLOD
10−4 (300 nl)	1.3 × 102 ± 4.4 × 101	1.0 × 102 ± 4.3 × 101	6.7 × 102 ± 3.3 × 102	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD
10−5 (30 nl)	3.2 × 101 ± 1.5 × 101	<PLOD	2.9 × 102 ± 2.1 × 102	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD
10−6 (3 nl)	<PLOD	<PLOD	5.2 × 101 ± 7.9 × 101	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD

^aFIB, fecal indicator bacteria; MST, microbial source tracking; PLOQ, process limit of quantification; PLOD, process limit of detection.