TABLE 2.

Concentrations of fecal indicator bacteria, bacterial and viral microbial source tracking markers, and enteric viruses per 3 μl of DNA/RNA extracted from beach water samples seeded with secondary-treated wastewater

Dilution and corresponding amt of secondary-treated wastewater into beach water samples	Concn (mean ± SD) (per 3 μl of DNA/RNA) ina:
	FIB (ENT 23S)	Bacterial or viral MST marker					Enteric virus
		EC H8	HF183	nifH	HAdV	PMMoV	HAdV 40/41	HNoV	EV
150 ml	2.4 × 103 ± 7.5 × 101	5.8 × 103 ± 3.8 × 102	2.0 × 104 ± 7.6 × 102	2.2 × 102 ± 7.0 × 101	2.1 × 101 ± 8.7 × 100	3.6 × 103 ± 3.9 × 102	<PLOQ	<PLOD	2.9 ×101 ± 1.1 × 101
10−1 (15 ml)	1.6 × 103 ± 4.9 × 102	1.3 × 103 ± 2.3 × 102	4.7 × 103 ± 9.4 × 102	3.5 × 101 ± 9.3 × 100	3.7 × 100 ± 3.0 × 100	4.8 × 102 ± 2.4 × 101	<PLOD	<PLOD	<PLOQ
10−2 (1.5 ml)	8.7 × 102 ± 7.4 × 102	2.7 × 102 ± 2.2 × 102	6.3 × 102 ± 4.0 × 102	<PLOD	<PLOD	1.8 × 102 ± 3.8 × 101	<PLOD	<PLOD	<PLOD
10−3 (150 μl)	9.4 × 102 ± 9.6 × 102	1.8 × 102 ± 2.0 × 102	3.3 × 102 ± 3.5 × 102	<PLOD	<PLOD	1.6 × 101 ± 1.5 × 101	<PLOD	<PLOD	<PLOD
10−4 (15 μl)	8.5 × 101 ± 5.0 × 101	1.5 × 101 ± 1.7 × 101	8.6 × 101 ± 1.4 × 102	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD
10−5 (1.5 μl)	<PLODa	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD
10−6 (150 nl)	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD	<PLOD

^aFIB, fecal indicator bacteria; MST, microbial source tracking; PLOQ, process limit of quantification; PLOD, process limit of detection.