FIG 3.

RT-PCR analysis of the transcription of genes in the rif cluster in different A. mediterranei strains. No PCR bands were observed when total RNA without reverse transcription was employed as the template, which indicated that the RNA samples were not contaminated by genomic DNA (data not shown). WT, wild type; KO, rifZ knockout mutant; NC, KO strain integrated with pDZL803, a negative control; C, KO strain integrated with pDZL8031, a complementation strain; M, DL1000 DNA ladder; +, the rpoB gene, used as an internal control. The analyzed genes were in the same order as shown in Fig. 1, where “S” stood for rifS, 35 stood for orf35, etc.